hsp70 3a3  (Santa Cruz Biotechnology)

Journal: Fish and Shellfish Immunology Reports

Article Title: The effects of Pandanus tectorius leaf extract on the resistance of White-leg shrimp Penaeus vannamei towards pathogenic Vibrio parahaemolyticus

doi: 10.1016/j.fsirep.2023.100101

Figure Lengend Snippet: Primer sequences, size (base pairs) and melting temperatures (Tm) used to amplify immune-related genes (Hsp70, crustin, prophenoloxidase, penaeidin, peroxinectin and transglutaminase) of P. vannamei .

Article Snippet: The PVDF membrane was probed with a 1:5000 dilution of a mouse monoclonal antibody to Hsp70 (Clone 3A3, Thermo Scientific, USA).

Techniques: Sequencing

Journal: Fish and Shellfish Immunology Reports

Article Title: The effects of Pandanus tectorius leaf extract on the resistance of White-leg shrimp Penaeus vannamei towards pathogenic Vibrio parahaemolyticus

doi: 10.1016/j.fsirep.2023.100101

Figure Lengend Snippet: The relative density (RD) of Hsp70 expression in P. vannamei incubated 24 h with P. tectorius leaf extract. The relative Hsp70 quantity was calculated based on the RD of non-exposed control, with a relative value of 1. Asterisks (*) indicate protein up-regulation.

Article Snippet: The PVDF membrane was probed with a 1:5000 dilution of a mouse monoclonal antibody to Hsp70 (Clone 3A3, Thermo Scientific, USA).

Techniques: Expressing, Incubation, Control

Journal: Fish and Shellfish Immunology Reports

Article Title: The effects of Pandanus tectorius leaf extract on the resistance of White-leg shrimp Penaeus vannamei towards pathogenic Vibrio parahaemolyticus

doi: 10.1016/j.fsirep.2023.100101

Figure Lengend Snippet: The relative fold-difference of immune-related genes of P vannamei upon 24 h incubation in 6 g/L Pandanus tectorius leaf extract. The value represents the fold changes of mRNA expression (normalized against internal control β-actin) relative to the control value, which are the samples from shrimp without any treatment. * represents significance difference between treatment and control (P < 0.05 ) . The data shown are representative of two independent experiments with similar expression patterns.

Article Snippet: The PVDF membrane was probed with a 1:5000 dilution of a mouse monoclonal antibody to Hsp70 (Clone 3A3, Thermo Scientific, USA).

Techniques: Incubation, Expressing, Control